Mustapha Aouida | Hamad Bin Khalifa University
Hamad Bin Khalifa University

FACULTY BIOGRAPHIES

Mustapha Aouida

Mustapha Aouida (PhD)


Research Scientist & Lab Manager
College of Health and Life Sciences

  • Phone+974 - 44548040
  • Office locationC-139, First Floor, LAS Building

Biography

Dr. Mustapha Aouida received his PhD in Molecular and Cell Biology in 2003 from both the Science University of Tunis and Gustave Roussy Institute, Paris. For his post-doctoral fellowship he joined Professor Ramotar at Maisonneuve-Rosemont Research Institute in Montreal where he studied the mechanism by which yeast cells and mammalian cells become resistant to the antitumor drug bleomycin. Dr. Aouida joined the College of Health and Life Sciences in April 2016 as a Lab Manager and is currently a Research Scientist at the college.

 


Research Interests

Genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated 9 (Cas9) technology is revolutionizing the study of gene function and likely will give rise to an entire new class of therapeutics for a wide range of diseases. Achieving this goal requires not only characterization of the technology for efficacy and specificity but also optimization of its delivery to the target cells. My laboratory is particularly interested to improve the efficiency and the specificity of CRISPR/Cas9 system and also to investigate the use of this technology in some applications such as cancer drug target discovery and understanding the molecular basis of diabetes and obesity.

Experience

Research Scientist

College of Health and Life Sciences

2019 - Present
  • Lab Manager

    Division of Life Sciences, College of Science and Engineering, Hamad Bin Khalifa University

    2016 - 2018
  • Research Scientist

    Genome Engineering Laboratory, King Abdullah of Science and Technology

    2010 - 2015
  • Postdoctoral Research Scientist

    Maisonneuve Research Center, University of Montreal

    2003 - 2010

Education

PhD in Biology (Molecular and Cell Biology)

Gustave Roussy Institute, Paris (France); Science University Of Tunis, Tunisia

2003
  • Master Degree in Microbiology

    Science University Of Tunis, Tunisia

    1999
  • Bachelor in Natural Sciences

    Bachelor in Natural Sciences

    1997
  • High School diploma in Science

    Technical High School Zarzis, Tunisia

    1993

Selected Publications

  • Mustapha Aouida and Abdelmoez Eshrif and Dindial Ramotar

    Yeast lacking the PP2A phosphatase regulatory subunit Rts1 sensitizes rad51 mutants to specific DNA damaging agents. Front. Genet. 2019 Nov 8;10:1117. doi: 10.3389/fgene.2019.01117. eCollection 2019

    2019
  • Mustapha Aouida and Dindial Ramotar

    The Budding Yeast Saccharomyces Cerevisiae as A Model System for Anti-Cancer Drug Screening. Clinics of Oncology 1 (6) 1-3.

    2018
  • Mustapha Aouida and Dindial Ramotar

    Identification of essential yeast genes involved in polyamine resistance. Gene https://doi.org/10.1016/j.gene.2018.08.066 In press

    2018
  • Mustapha Aouida, Ayman Eid, and Magdy Mahfouz

    CRISPR/Cas9-mediated target validation of the splicing inhibitor Pladienolide B. Biochimie Open. 2016 Feb 24;3:72-75. doi: 10.1016/j.biopen.2016.02.001. eCollection 2016 Dec.

    2015
  • Mustapha Aouida, Ayman Eid, Zahir Ali, Thomas J. Cradick, Harshavardhan Deshmukh, Ahmed Atef, Dina B. AbuSamra, Ciaran M. Lee, Samah Zeineb Gadhoum, Jasmeen S. Merzaban, Gang Bao and Magdy M. Mahfouz

    Generation of an Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering. PLoS One. 2015 Jul 30;10(7):e0133373. doi: 10.1371/journal.pone.0133373. eCollection 2015.

    2015
  • Zahir Ali, Aala Abul-faraj, Lixin Li, Neha Ghosh, Marek Piatek, Ali Mahjoub, Mustapha Aouida, Agnieszka Piatek, Nicholas J. Baltes, Daniel F. Voytas, Savithramma Dinesh-Kumar, and Magdy M. Mahfouz

    Efficient Viral-Mediated Genome Editing in Plants using the CRISPR/Cas9 System (2015). Mol Plant. 2015 Mar 6. pii: S1674-2052(15)00162-8. doi: 10.1016/j.molp.2015.02.011

    2015
  • Mustapha Aouida, Lixin Li; Ali Mahjoub, and Magdy M. Mahfouz

    The use of transcription activator- J Biosci Bioeng. 2015 Apr 20. pii: S1389-1723(15)00095-X. doi: 10.1016/j.jbiosc.2015.02.017

    2015
  • Piatek, Agnieszka; Ali, Zahir; Baazim, Hatoon; Li, Lixin; Abulfaraj, Aala; Al-Shareef, Sahar; Aouida, Mustapha; Mahfouz, Magdy

    RNA-guided transcriptional regulation in planta via synthetic dCas9- based transcription factors. Plant Biothechnology 2015 May; 13(4):578-89.

    2015
  • Mustapha Aouida, Marek J. Piatek, Dhinoth K. Bangarusamy and Magdy M. Mahfouz

    Activities and Specificities of homodimeric TALENs in Saccharomyces cerevisiae. Current genetics (2013). In press

    2013
  • Mustapha Aouida, Kangchang Kim, AbdulRajjak S Shaikh, Jose M. Pardo, Jorg Eppinger, Ray A. Bressan, Meena L. Narasimhan

    A split luciferase method to identify Adiponectin receptors agonists using Saccharomyces cerevisiae as a model organism. PLoS One. 2013 Jun 7;8(6):e65454. doi: 10.1371/journal.pone.0065454. Print 2013

    2013
  • Mustapha Aouida, Marta Rubio Texeira, Johan M. Thevelein, Richard Poulin and Dindial Ramotar

    AGP2 Encodes a Multifunctional Transceptor that Positively Regulates Polyamine Transport. PLoS One. 2013 Jun 3;8(6):e65717. doi: 10.1371/journal.pone.0065717. Print 2013

    2013
  • Li, Lixin; Atef, Ahmed; Piatek, Agnieszka; Ali, Zahir; Piatek, Marek; Aouida, Mustapha; Sharakou, Altan; Mahjoub, Ali; Wang, Guangchao; Khan, Suhail; Fedoroff, Nina; Mahfouz, Magdy

    Characterization and DNA Binding Specificities of Ralstonia TAL-like Effectors. Mol Plant. 2013 Jan 8.

    2013
  • Dindial Ramotar and Mustapha Aouida. Cellular protection against the antitumor drug bleomycin

    Book Chapter, DNA repair in cancer therapy Cancer Drug Discovery and Development (2012) Edited by Lawrence C. Panasci and Moulay A. Alaoui-Jamali In Press.

    2012
  • Mulangi, Vipaporn Phuntumart, Mustapha Aouida, Dindial Ramotar and Paul Morris

    Functional Characterization of Osput1 A Spermidine Preferential Transporter In Rice. Planta (2012) Jan;235(1):1-11.

    2012
  • Mustapha Aouida and Dindial Ramotar

    A potential transporter to revamp therapy with the anticancer drug bleomycin. Curr Drug Metab. 2010 Sep; 11(7):595-602.

    2010
  • Wehmeier S, Arnold MF, Marlow VL, Aouida Mustapha, Myka KK, Fletcher V, Benicasa M, Scocchi M, Ramotar D, Ferguson GP

    Internalization of a thiazole-modified peptide in Sinorhizobium meliloti occurs by BacA – dependent and independent mechanisms. Microbiology, 2010 Sep; 156(Pt 9): 2702 -13

    2010
  • Mustapha Aouida, Richard Poulin, and Dindial Ramotar

    New Gateway to Treat Leukemia and Other Cancers, Science Daily (Mar, 27, 2010). http:// www.sciencedaily.com/releases/2010/03/100325113425.htm

    2010
  • Mustapha Aouida, Richard Poulin, and Dindial Ramotar

    The Human Carnitine Transporter SLC22A16 Mediates High Affinity Uptake of the Anticancer Polyamine Analogue Bleomycin-A5 J. Biol. Chem. 2010 285: 6275-6284

    2010
  • Ali kodami-Pour, Mustapha Aouida and Dindial Ramotar

    The Na+/H+ Exchanger Nhx1 of Saccharomyces cerevisiae is Essential to Limit Drug Toxicity. Advanced Studies in Biology, Vol. 2, 2010, no. 1, 15 – 34

    2010
  • Mustapha Aouida, Ali kodami-Pour and Dindial Ramotar

    Novel role for the Saccharomyces cerevisiae oligopeptidetransporter Opt2 in vacuolar assembly and drug detoxification. Biochem and Cell Biology 2009: Vol 87 653-661.

    2009
  • Mustapha Aouida and Dindial Ramotar

    A potential mechanism of resistance to the anticancer drug bleomycin. In: Mohan RM, editor. Research Advances in Cancer: Vol., Part, Kerala, India, Global Research Network 247-260, 2007.

    2007
  • Mustapha Aouida, Halima Mekid, Omrane Belhadj Lluis M. Mir and Omar Tounekti

    Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the antitumor agent bleomycin in S. cerevisiae. (2007) Biochemistry and Cell Biology. Vol. 85 No. 1 Pages 49-55.

    2007
  • Mustapha Aouida and Dindial Ramotar

    Bleomycin transport holds the key for improved anticancer therapy. Review Article. (2006). Cancer Therapy Vol 4, 171-182.

    2006
  • Mustapha Aouida, Kaouther Tounekti, Anick Leduc, Jeremie Poschmann, Xiaoming Yang, Omrane Belhadj, and Dindial Ramotar

    A defect (spt10) in chromatin remodeling sensitizes yeast cells to a subclass of DNA damaging agents. (2006). Environ Mol Mutagen. 47(9):707-17.

    2006
  • Mustapha Aouida, Anick Leduc, Richard Poulin and Dindial Ramotar

    AGP2 encodes the major permease for high affinity polyamine import in Saccharomyces cerevisiae. J Biol Chem. (2005): 280(25): 24267-24276

    2005
  • Mustapha Aouida, Omar Tounekti, Anick Leduc, Lluis M. Mir and Dindial Ramotar

    Isolation and characterization of Saccharomyces cerevisiae mutants with enhanced resistance to the anticancer drug bleomycin. Current Genet. 2004 May; 45(5):265-72

    2004
  • Mustapha Aouida, Nicolas Page, Anick Leduc, Matthias Peter and Dindial Ramotar

    A genome-wide screen in Saccharomyces cerevisiae reveals altered transport as a major mechanism of resistance to the anticancer drug bleomycin. Cancer Res. 2004 Feb 1; 64(3):1102-9.

    2004
  • Mustapha Aouida, Huijie Wang, Anick Leduc, and Dindial Ramotar

    Characterization of the transport and detoxification pathway for the antitumor drug bleomycin in Saccharomyces cerevisiae. Biochem J. 2004 Nov 15; 384(Pt 1):47-58.

    2004
  • Belhadj Zouhour, Mustapha Aouida and Omrane Belhadj

    Etude comparative de la cytotoxicité de deux antibiotiques antitumoraux, la bléomycine et laphléomycine, sur trois souches de la levure Saccharmyces cerevisiae. Microbiologie Hygiene et Alimentaire Vol. 16 :46 (2004) : 44-50.

    2004
  • Mustapha Aouida, Omar Tounekti, Omrane Belhadj and Lluis M. Mir

    Comparative roles of cell wall and cell membrane in limiting xenobiotic molecules uptake by Saccharomyces cerevisiae. Antimicrobial Agents and Chemotherapy 2003 Jun; 47(6):2012-4.

    2003
  • Mustapha Aouida, Omar Tounekti, Lluis M. Mir and Omrane Belhadj

    Yeast excellent model to study apoptosis. Microbiologie Hygiene et Alimentaire Vol.15 :42 (2003).

    2003