Control of Pluripotent Stem Cell States by Long-range Enhancer-promoter Contacts Enhancers are gene regulatory elements dispersed throughout the noncoding genome that control spatiotemporal gene expression programs during mammalian development. Enhancers can be located at considerable genomic distances to the target genes they regulate (in some cases hundreds of kilobases) and they can skip over more proximally located genes to regulate their target genes through direct physical contacts. This is crucial for normal development, as is evident from the finding that aberrant enhancer-promoter contacts can lead to developmental disorders.
Promoter Capture Hi-C (PCHi-C) is a method that maps enhancer-promoter contacts genome-wide at high resolution.
This webinar will highlight how PCHi-C is used to interrogate the dynamics of enhancer-promoter contacts during cellular differentiation and in response to developmental stimuli, with a focus on mouse and human pluripotent stem cell models. The research currently focuses on understanding how genetic variants in enhancers impact the gene regulatory landscape and how they shape inter-individual differences in human induced pluripotent stem cell differentiation potential.
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