Core Facilities

The Genomics Core Facility at QBRI actively supports scientific research by providing advanced research technologies and services to the research community at HBKU and QBRI, and their collaborators from academia and industries to address the complex biomedical problems of QBRI’s research interests in the field of cancer biology, metabolic disorders and neurological diseases.

Genomics Core and Genome Technology

The Genomics Core and Genome Technology laboratory is equipped with various state-of-the-art, high-throughput technological platforms to sequence the nucleic acids by next-generation sequencing (NGS) as well by capillary sequencing methods; as well as capillary sequencing methods and different array platforms. Our applications include sequencing of different nucleic acid templates, high throughput genotyping, epigenome analysis, gene expression analysis, single-cell technologies, NanoString technologies and digital PCR methods.

The Genomics Core and Genome Technology laboratory operation is aligned to support the vision and mission of QBRI-based fundamentals; assisting the researchers with design of an experiment, providing intellectual support and novel method development for genomics experiments; interface with other core technological platforms to facilitate integrated biology research; and collaborative research.

Resources and Services

Facilities and Capabilities

The facilities and instruments that are available at QBRI’s Genomics Core Facility are classified broadly under the following categories

Next Generation Sequencing

  • Illumina HiSeq 4000 and 2500 platforms (high throughput sequencing for whole exome sequencing , and RNA sequencing “total RNAseq, mRNAseq, miRNAseq, 3′-Tag-Seq,…”)
  • Illumina MiSeq (for smaller genome, amplicon sequencing and targeted sequencing)

Large Scale Genotyping Analysis

  • Illumina iScan array scanner (Illumina Bead Array technology for high-throughput SNP genotype analysis)
  • ABI 3730xl 96 capillaries, ABI 3500xl 24 capillaries and ABI 3100xl 16 capillaries Genetic Analyzers (Sanger sequencing)
  • NanoString Technology (Copy number variation analysis of up to 800 target regions)

Targeted Gene Expression Analysis

  • NanoString Technologies (targeted gene expression for up to 800 targets “mRNA expression, long non coding lncRNA expression, Leukemia fusion gene analysis and miRNA expression)
  • QuantStudio 12K Plus for qPCR and Digital PCR and Gene Expression Panels studies
  • ABI 7900 Real-Time PCR System

Single Cell Genomics

  • Fluidigm C1 Single-Cell Auto Prep (single-cell mRNA sequencing, single-cell target gene expression, Single-cell miRNA expression and single-cell whole exome sequencing)
  • Bio-Rad ddSEQ System and NGS platform (single cell RNA sequencing)

DNA methylation Analysis

  • Illumina HiSeq 4000 platform (Methylated DNA immunoprecipitation sequencing “MeDIP-Seq”, whole-genome bisulfite sequencing and amplicon methyl-seq or target enrichment)
  • Illumina iScan array scanner (methylation profiling microarray of over 850,000 methylation sites)
  • Core Laboratory Services

    • Advice on experimental design and sample preparation
    • Quality control of nucleic acid materials by LabChip and Bioanalyzer
    • Different types of sequencing library preparation and data generation by high-throughput sequencing methods
    • Quantitative PCR for differential gene expression analysis
    • Helping researchers with the generation of preliminary data for grant applications
    • Data management and storage

    Major Instruments and Services

    Major Instruments
    • Illumina HiSeq 4000, 2500 and Miseq
    • Capillary Sequencers
    • Illumina Iscan
    • Nanostring System
    • QuantStudio 12K Flex
    Major Services
    • Experimental Design
    • Whole Genome, Transcriptome, and Exome Sequencing
    • Targeted Gene
    • Expression Studies
    • Genotyping Studies