Core Facilities

The Flow Cytometry Core Facility at QBRI is a key facility within HBKU’s research landscape and is equipped with state-of-the art flow cytometry hardware, software and intellectual capabilities.

Flow Cytometry Core

The Flow Cytometry Core very actively interacts with all research teams and the other core facilities to address reliable, accurate and innovative solutions in line with HBKU’s focus on cancer research, metabolic disorders and neurological diseases. In that sense, the Flow Cytometry Core serves as a veritable Shared Resource Lab (SRL), providing its instrumentation, infrastructure and knowledge to optimize the scientific output for any flow-related project within HBKU.

The mission of the QBRI Flow Cytometry Core is to offer researchers efficient, reliable and innovative flow cytometry and cell sorting solutions with the highest standards of instrumentation, quality control, biosafety and productivity. This is achieved by providing access to state-of-the-art instrumentation and professional flow cytometry and cell sorting services and is supported by a highly motivated staff with extensive skills and profound expertise in the field of flow cytometry. Recently established scientific collaborations include specific research teams at QBRI, such as the cancer and the stem cell research teams, as well as the advanced imaging and genomics cores.

The collaborations aim to develop, evaluate, establish and optimize advanced flow cytometry and single-cell sorting applications. As a vivid player and innovative flow cytometry hub in Qatar and beyond, the QBRI Flow Cytometry Core is also in collaborative exchange with partners from industry (e.g. BD, sedeer) and external research institution (e.g. QCM-Q, HBKU, HMC). As such, the flow cytometry core assists with experimental design, sample preparation, data acquisitions and analysis. In addition, the QBRI Flow Cytometry Core also provides support and guidelines with regards to flow cytometry education, operation of instruments, software workflows and biosafety to fulfill the mission of a fruitful, collaborative and highly innovative environment for research and development. To that aim, the QBRI Flow Cytometry Core started to establish its education and knowledge transfer channels by organizing and hosting a very successful flow cytometry symposium in May 2017 and has offered several training and laboratory courses for QBRI and HBKU staff and students since then. Qatar Biomedical Research Institute (QBRI)

Resources and Services

Equipment

  • BD Accuri C6 flow cytometer (2 lasers, 6 detectors)
  • BC Gallios flow cytometer (3 lasers, 12 detectors)
  • BD LSR Fortessa X-20 SORP flow cytometer (5 lasers, 20 detectors)
  • Amnis Image Stream MKII imaging flow cytometer (2 lasers, 6 detectors)
  • CellSee CTC Single Cell Analysis System
  • 2 x BD FACSAria SORP cell sorter (5 lasers, 18 detectors)
  • BD FACSJazz cell sorter (3 lasers, 8 detectors, in BSL-2 cabinet)
  • Flow cytometry software analysis (BD FACSDiva, FlowJo, BD Sortware, ModFit LT, etc.)

Services

  • Advice on sample preparation (reagent selection, protocol design & optimization)
  • Advice and support on multicolor panel design (negative controls, compensation setup, and multicolor panels)
  • Multicolor sample acquisition and analysis
  • Single cell analysis and sorting applications
  • Aseptic cell sorting and biosafety advice
  • Surface phenotyping and intracellular staining
  • Apoptosis, cell cycle analysis, and proliferation
  • Flow cytometry training and education of researchers and students
  • Support with data analysis and presentation
  • Data management and storage

Major Instruments and Services

Major Instruments
  • Flow Analyzer
  • Cell Sorter
  • Image Stream
  • CTC System
Major Services
  • Flow Analysis
  • Bulk Cell Sorting
  • Single Cell Sorting
  • Experimental Design
  • Data Analysis
  • Data Management
  • Training